In view of the aforementioned observations and considerations we hypothesized
Concomitant D1-Ser209Ala and D1- Ser212Cys mutations may improve the functional stability of PSIIRC in mesophilic cyanobacteria grown at elevated temperatures. We further hypothesized that enhanced concentration should compensate for the increased Rubisco affinity to oxygen at elevated temperatures and thereby reduce the impairment of the D1 repair mechanism. PCC 6803 which was selected as control because it retains only the intact wild-type psbAII gene followed by a kanamycin resistance gene. The absence of the other two gene copies, psbAI and III genes, which are replaced by spectinomycin and chloramphenicol LY2835219 CDK inhibitor resistance cartridges, simplifies the interpretation of genetic modifications in the D1 protein subunit. There was no significant difference in the growth rates and pigmentation between the two strains when grown at 30uC under normal air bubbling. When incubated at the growth of mutant was slightly slower, in comparison to the control strain that showed a much slower growth at both temperatures. Importantly, when grown at the same temperature but under stirring the AC biomass Reversine Aurora Kinase inhibitor increased at relatively slow pace and started to level off at the fourth day. Nevertheless, when transferred back to 30uC the growth was regained. In contrast, under the same conditions, the DKS cultures leveled off after three days of slow growth and could not recover when transferred back to 30uC.
In view of the aforementioned observations and considerations we hypothesized Concomitant D1-Ser209Ala and D1- Ser212Cys
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