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Lipid classes were separated by thin-layer chromatography, sprayed with a primuline solution and quantified by integrating variable pixel intensities of the scanned plates. The values represent the mean of three Paclitaxel Microtubule inhibitor independent experiments. The deviation of values was within 6 2%. Monogalactosyldiacylglycerol, MGDG; digalactosyldiacylglycerol, DGDG; phosphatidylglycerol, PG; sulfoquinosyldiacylglycerol, SQDG. Introduction In prokaryotes translation of mRNA is coupled to transcription whereas in eukaryotes it takes place in the cytosolic compartment, separated by the nuclear membrane from the transcriptionally active chromosomes. The eukaryotic translation process has thus been Pazopanib VEGFR/PDGFR inhibitor considered to be entirely independent of transcription. Transcription of protein-encoding genes is controlled by two types of DNA elements, enhancer and core promoter. The gene specific enhancer elements serve as binding sites for transcription regulatory factors and can be divided into two classes: those that function independently of their position relative to the transcription start site and those that can activate transcription only when located proximal to the TSS.
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